PADI2 Stable Expression Cell Line (HCT 116)

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  • 细胞图0 PADI2 Stable Expression Cell Line (HCT 116)-细胞图0
  • 细胞图1 PADI2 Stable Expression Cell Line (HCT 116)-细胞图1
  • 质粒图0 PADI2 Stable Expression Cell Line (HCT 116)-质粒图0
  • PADI2 Stable Expression Cell Line (HCT 116) PADI2 Stable Expression Cell Line (HCT 116)-产品图
细胞图0 PADI2 Stable Expression Cell Line (HCT 116)-细胞图0
细胞图1 PADI2 Stable Expression Cell Line (HCT 116)-细胞图1
质粒图0 PADI2 Stable Expression Cell Line (HCT 116)-质粒图0
PADI2 Stable Expression Cell Line (HCT 116) PADI2 Stable Expression Cell Line (HCT 116)-产品图
PADI2 Stable Expression Cell Line (HCT 116) Copy Product Info
Catalog#： YOE-L019
Size： 1*10^6
Culture method： 90%McCOY's 5A+10% FBS
STR Report
Datasheet (Use Instruction)
  • 细胞图0 PADI2 Stable Expression Cell Line (HCT 116)-细胞图0
  • 细胞图1 PADI2 Stable Expression Cell Line (HCT 116)-细胞图1
  • 质粒图0 PADI2 Stable Expression Cell Line (HCT 116)-质粒图0
  • PADI2 Stable Expression Cell Line (HCT 116) PADI2 Stable Expression Cell Line (HCT 116)-产品图
PADI2 Stable Expression Cell Line (HCT 116) Copy Product Info
Catalog#： YOE-L019
Size： 1*10^6
Culture method： 90%McCOY's 5A+10% FBS
Datasheet (Use Instruction)

Detailed Product Information

Cell Info

Catalog YOE-L019 Transcript NM_007365.3
Cell name PADI2 Stable Expression Cell Line (HCT 116) Species Human
Gene Name PADI2 Passage ratio 1:2~1:4
Cell Morphology Epithelial-like, adherent Antibiotic Concentration Puro=0.5μg/ml
Culture method 90%McCOY's 5A+10% FBS Mycoplasma test negative
Catalog
YOE-L019
Cell name
PADI2 Stable Expression Cell Line (HCT 116)
Gene Name
PADI2
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Transcript
NM_007365.3
Species
Human
Antibiotic Concentration
Puro=0.5μg/ml
Mycoplasma test
negative
Culture method
90%McCOY's 5A+10% FBS

Product Description

This gene encodes a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. The family members have distinct substrate specificities and tissue-specific expression patterns. The type II enzyme is the most widely expressed family member. Known substrates for this enzyme include myelin basic protein in the central nervous system and vimentin in skeletal muscle and macrophages. This enzyme is thought to play a role in the onset and progression of neurodegenerative human disorders, including Alzheimer disease and multiple sclerosis, and it has also been implicated in glaucoma pathogenesis. This gene exists in a cluster with four other paralogous genes.
Gene Expression Validation
No. Sample hPADI2-1CтMean β-h_actin CтMean ΔCтMean ΔΔCт Expression Rate
1 PADI2 Stable Expression Cell Line (HCT 116) 19.474 14.147 5.327 -8.521 367.347
2 HCT116_Ctrl(Puro) 29.721 15.873 13.848 0.000 1.000
STR Profile
Download STR Report
Loci STR Info (Sample Cell)
Sample Cell Line:HCT 116		 STR Info (Cell bank)
Cell Line:HCT 116
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 10111011
D13S317 10121012
D7S820 11121112
D16S539 11131113
VWA 17221717
TH01 8989
AMEL XXXY
TPOX 8889
CSF1PO 710710
D12S391 1721
FGA 1823
D2S1338 1616
D21S11 2930
D18S51 1617
D8S1179 1214
D3S1358 1219
D6S1043 1313
PENTAE 1314
D19S433 1213
PENTAD 913
D1S1656 1214
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • icon
    High expression
    Exclusive cell selection technique, ensuing high expression, RT-qPCR validation data provided.
  • icon
    Good stability
    Stable gene copy number, low passage, can be stably passaged for more than 20 passages.
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    Strict QC
    Strict quality control, ensuing no bacterial and mycoplasma contamination, STR authentication and cell post-thawing viability test passed; High standard delivery.
Citations:
Product Catalog#：YOE-L019
Product Name：PADI2 Stable Expression Cell Line (HCT 116)
Related Resources: Red Cotton Gene-editing System | ATCC | DSMZ | ECAZZ | Cellosaurus | CCTCC | NCBI
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